|     Pregnancy   Pregnancy Length - Dogs: 
  
    Highly variable
  
    58-68 days from last breeding.
  
    57 days from first day of
    diestrus.
  
    64-66 days from the Luetenizing hormone peak
    blood levels   Implantation   Pregnancy Diagnosis
Techniques 
1.  Palpation of the Abdomen 
  
    Best done at 20-30 days because distinct
    round masses are more easily palpated.
  
    Pregnancy diagnosis after >30 days is
    difficult because the uterus becomes diffusely enlarged.
  
    Pregnancy diagnosis is possible again after
    about day 50 because individuals can again be palpated.
  
    Pregnancy detection by palpation is an art;
    difficult to do. Limited by the temperament of the female, obesity,
    abdominal tenseness, size of the female, small litter size, etc. 2.  Radiography (X-ray) 
  
    Useful in diagnosis of pregnancy, predicting
    dystocia, and giving a rough idea of littler size.
  
    By day 21-42 fluid filled uterine horns may
    be detected.
  
    After day 42 various degrees of fetal
    ossification (bone formation) may be detected. 3.  Ultrasound 4.  Mammary engorgement 
  
    An inconsistent indicator of pregnancy.
  
    Many females will not display mammary
    development till near term, especially primiparous females. 5.  Behavior 
  
    Many pregnant females will show decreased
    appetite – sometimes even total anorexia – for a few days during either
    the third or fourth week of pregnancy.
  
    This seems to be a fairly reliable, if
    non-specific, indicator of pregnancy. TABLE 2.The techniques to determine pregnancy in the bitch and the range through which
the procedure is accurate. The days listed are after the luteinizing hormone (LH) peak.
 
  
    
      | 
          Method         | 
          First Day         | 
          Last Day         | 
          Effectiveness         |  
      | 
          Ultrasonography         | 
          18         | 
          Birth         | 
          1         |  
      | 
          Ultraonography(fetal Heart Beat)
 | 
          24         | 
          Birth         | 
          2         |  
      | 
          Radiology(X-ray)
 | 
          45         | 
          Birth         | 
          3         |  
      | 
          Relaxin Assay         | 
          25 (30)         | 
          Birth         | 
          ??         |  
      | 
          Acute phase protein assay (experimental)         | 
          40         | 
          Birth         | 
          not practical         |  References: 1. A study guide to small animal reproduction.
Barton & Herron. Texas A & M University |  |